Lab Protocols

From LA Biohackers

This is the central place for storing our lab protocols. Link to other wiki article or upload pdf.

Contents 1 General Protocols 2 Molecular Bio Protocols 3 Sequencing 4 PCR Protocols 5 Microbiology Protocols 6 Bioinformatics Protocols 7 Cell culture Protocols 8 Electrophys Protocols 9 Chemistry Protocols 10 awesome links to yet more protocols in descending awesomeness

General Protocols

• Buffers, gels and solutions (PDF)
• Basic protocols Media:PradeLabProtocol.pdf, from the Prade Lab at Oklahoma State University

Molecular Bio Protocols

• Plasmid DNA prep
• digestion
• ligation
• Electrophoresis
  • quantitation
  • ladder
• PCR
  • noob tutorial
  • designing your primers
  • basic pcr protocol
• restriction enzyme database >REBASE

Sequencing

• See main Gene Sequencer page

PCR Protocols

• See main PCR Protocols page

Microbiology Protocols

• Genomikon Lab Manual -- a few good starter experiments

Bioinformatics Protocols

• See main Bioinformatics page

Cell culture Protocols

Electrophys Protocols

Chemistry Protocols

• TLC Plate Preparation

awesome links to yet more protocols in descending awesomeness

• http://www.protocol-online.org/
• http://info.med.yale.edu/genetics/ward/tavi/databases.html/